From season 2 of The Lone Ranger cartoon:
Tonto goes it alone to end "The Reign of the Queen Bee". Some question as to the actress voicing Queen Bee. A comment thread on YouTube suggests June Foray. I'd otherwise suspect Agnes Moorhead (Bewitched), who had appeared in two episodes as Black Widow. If anyone knows for sure, feel free to contribute.
Forgive the video quality.